p-nitrophenyl phosphate assay formula

4

4-Nitrophenol is used to manufacture drugs fungicides insecticides and dyes and to darken leather Acute (short-term) inhalation or ingestion of 4-nitrophenol in humans causes headaches drowsiness nausea and cyanosis (blue color in lips ears and fingernails) Contact with eyes causes irritation in

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extinction coefficient of p nitrophenol

p-Nitrophenyl Phosphate (PNPP) is a non-proteinaceous non-specific substrate used to assay protein alkaline and acid phosphatases The PNPP phosphatase activity is measured using a continuous or single-point spectrophotometric assay based on the ability of phosphatases to catalyze the hydrolysis of PNPP to p-nitrophenol a chromogenic product

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4

CAS: 4264-83-9 CAS ALT: 71735-29-0 MDL: MFCD00066288 Synonyms: di-Sodium 4-nitrophenyl phosphate hexahydrate 4-Nitrophenyl phosphate disodium salt hexahydrate p-Nitrophenyl phosphate disodium salt hexahydrate pNPP Phosphatase substrate CAS Number: 333338-18-4 MDL Number: MFCD00066288 Molecular Formula: C6H6NO6P Molecular Weight: 263 05 br

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Product Name: 4

Product Name: 4-NITROPHENYL PHOSPHATE disodium salt hexahydrate LR Chemical Formula: Product Code: NL077 CAS No 4264-83-9 Molecular Weight: 371 14 Properties Description Assay: 99 0% min UN Number: None Allocated Quality Specification Class: None Allocated Hazchem code: None Allocated Poison schedule: Not Scheduled General Information: Store

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4

4-Nitrophenyl phosphate disodium salt hexahydrate is a chromogenic substrate yielding a soluble yellow chromophor for the colorimetric assay of phosphatases and is used for determining alkaline phosphatase activity in human serum Product Specifications4-Nitrophenyl phosphate disodium salt hexahydratePNPP p-nitrophenyl phosphate para nitrophenyl phosphate PNP-phos 2 Na

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P

This p-Nitrophenyl Phosphate is validated for use in ELISA Excellent substrate for alkaline phosphatase-based ELISA assays Produces yellow soluble end product that can be read at 405 nm to 410 nm Both kinetic and endpoint determinations can be performed Supplied as ready-to-use

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4

Synonyms: p-Nitrophenyl phosphate disodium hexahydrate pNPP-2Na Purity Limit: ≥ 99% (Assay HPLC) Molecular Formula: C 6 H 4 NNa 2 O 6 P6H 2 O Molecular Weight: 371 10 CAS No: 4264-83-9 MDL No: MFCD00066288 Appearance: Off-white to pale yellow tinge crystalline powder Melting Point: 300 C Storage Temp: Store at ≤ -4 C

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Alkaline Phosphatase Kin 4+1 Red Logo

The series of reactions involved in the assay system is as follows: Alkaline phosphatase (ALP) hydrolyzes the colorless p-nitrophenyl phosphate to p-nitrophenol and phosphate in the presence of magnesium ions The product of enzyme hydrolysis p-nitrophenol has a yellow color at the pH of the reaction p-Nitrophenyl phosphate+H

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4

4-Nitrophenyl phosphate disodium salt hexahydrate or p-Nitrophenyl phosphate disodium salt hexahydrate Manufacturers SDS GHS MSDS Sheet 4-Nitrophenyl phosphate disodium salt hexahydrate or p-Nitrophenyl phosphate disodium salt hexahydrate: CAS Number: 4264-83-9 or 333338-18-4 EINECS EC Number: 224-246-5 Molecular Formula: C6H4NO6PNa2- 6H2O or

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Alkaline phosphatase

Alkaline phosphatase (ALP ALKP ALPase Alk Phos) (EC 3 1 3 1) or basic phosphatase is a homodimeric protein enzyme of 86 kilodaltons Each monomer contains five cysteine residues two zinc atoms and one magnesium atom crucial to its catalytic function and it is optimally active at alkaline pH environments ALP has the physiological role of dephosphorylating compounds

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4

Synonym: p-Nitrophenyl phosphate disodium hexahydrate Disodium 4-nitrophenyl phosphate hexahydrate di-Sodium 4-nitrophenyl phosphate pNPP disodium hexahydrate CAS Number: 333338-18-4 Empirical Formula (Hill Notation): C 6 H 4 NNa 2 O 6 P 6H 2 O Molecular Weight: 371 14 EC Number: 224-246-5 MDL Number: MFCD00066288 Linear Formula: O 2 NC 6 H 4 OP(O)(ONa) 2

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4

Nitrophenols include two chemicals 2-nitrophenol and 4-nitrophenol which are very similar to each other They are manufactured chemicals that do not occur naturally in the environment The manufacture of one almost always produces a little of the other so they are grouped together when discussing their properties and harmful effects

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OsPAP26 Encodes a Major Purple Acid Phosphatase and

To measure specific APase activity 1 μg (root and medium protein extracts) or 10 μg (leaf protein extracts) of proteins were added to 600 μl of pre-warmed 10 mM pNPP (p-nitrophenyl phosphate) in 50 mM sodium acetate at pH 5 5 Reactions were allowed to proceed for 30 min (root and leaf) or 10 min (medium) at 25 C and then stopped with 1 2

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Alkaline Phosphatase Assay Kit

The Alkaline Phosphatase Assay Kit is designed to measure ALP activity directly in biological samples without pretreatment The improved method utilizes p-nitrophenyl phosphate that is hydrolyzed by ALP into a yellow colored product (maximal absorbance at 405nm) The rate of the reaction is directly proportional to the enzyme activity p

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extinction coefficient of p nitrophenol

p-Nitrophenyl Phosphate (PNPP) is a non-proteinaceous non-specific substrate used to assay protein alkaline and acid phosphatases The PNPP phosphatase activity is measured using a continuous or single-point spectrophotometric assay based on the ability of phosphatases to catalyze the hydrolysis of PNPP to p-nitrophenol a chromogenic product

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pNPP Disodium Hexahydrate

bioWORLD pNPP Disodium Hexahydrate - pNPP Disodium Hexahydrate is the substrate of choice for use with alkaline phosphatase in Enzyme Linked Immunosorbant Assay (ELISA) procedures The reaction produces a soluble end product that is yellow in color pNPP Disodium Hexahydrate can be used to perform kinetic and endpoint determinations pNPP Disodium Hexahydrate is used as

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Alkaline Phosphatase (ALP)

ASSAY PROCEDURE Principle alkaline phosphatase p-Nitrophenyl phosphate H 2 O ⎯⎯⎯⎯⎯⎯⎯⎯⎯→p-Nitrophenol (PNP) H 3 PO 4 The appearance of p-nitrophenol is measured spectrophotometrically at 405 nm Definition of unit One unit (U) is defined as the amount of enzyme which produces 1 μmol of PNP per min at 37C and pH 9 8 under

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pNPP Disodium Hexahydrate 1 g

pNPP Disodium Hexahydrate is the substrate of choice for use with alkaline phosphatase in Enzyme Linked Immunosorbant Assay (ELISA) procedures The reaction produces a soluble end product that is yellow in color pNPP Disodium Hexahydrate can be used to perform kinetic and endpoint determinations pNPP Disodium Hexahydrate is used as

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