glucose sigma cell culture

Cell culture

cell culture: the maintenance or growth of animal cells in vitro or a culture of such cells tissue and glucose for the culture and isolation of Brucella melitensis It may be supplemented by the addition of sheep blood and vitamin K 1 solution for the isolation of anaerobic bacteria buffered charcoal yeast extract Sigma-Aldrich

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A multi

glucose limitation without running into glucose starvation and of the optimal harvest time Materials and methods Cell culture and fed-batch process Cell line A an in-house CHO DG44 cell line producing mAb A was used as model cell line in this study Cells were maintained in proprietary serum-free basal medium in shake flask at 37˚C 5% CO 2

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Glucose Uptake Measurement and Response to Insulin

Glucose uptake is a key biological measurement for testing activators or inhibitors on cell culture and how they impact glucose use and the ability of the cell to respond to insulin The method described here has been shown to be quick and reliable and has been widely used in many studies using primary

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WO2004104186A1

Methods of improving protein production in animal cell cultures are provided Cell culture methods are presented wherein glucose is fed in a restricted manner to cell culture this restricted feeding of glucose to the cell culture results in lactate production being controlled to a low level The restricted feeding of glucose in a fed-batch process is not accomplished through a constant-rate

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Tumour cell culture survival following glucose and

1 1 Tumour cell culture survival following glucose and 2 glutamine deprivation at typical physiological 3 concentrations 4 5 Edward Henry Mathews PhD a B Andr Stander PhD b 6 Annie M Joubert PhD b and Leon Liebenberg PhD a * 7 8 a Centre for Research and Continued Engineering Development North-West University 9 Suite No 91 Private Bag X30 Lynnwood Ridge 0040 South

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Cell Culture Media: A Review

So cell culture media should support maximal cell growth and sustain cell viability at increasing cell densities For the production of virus not just high cell densities are required but there must be abundant nutrients in the media to sustain virus replication after infection for example DMEM High Glucose H-21 for Lenti-X 293T cells [ 53 ]

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BioAssay Systems Glucose Uptake

Culture Starve and Treat Cells 1 Seed 100 L of 1-10 103 adherent cells (or 1-5 104 suspension cells) into each well of a 96-well culture plate Incubate for 4 hours or overnight at 37C in a cell culture incubator Note: The cell number to be used depends on cell size and metabolic demand of glucose 2

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High

9/29/2017Cell isolation culture and preparation of HDL HUVECs (Cell Applications Inc San Diego CA USA) were maintained in MCDB (Molecular Cellular and Developmental Biology) medium containing 15% fetal bovine serum (FBS lipoprotein deficient) 0 009% heparin 0 015% endothelial cell growth supplement ( Number E0760 Sigma St Louis MO

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Dipyridamole increases oxygen

Dipyridamole Increases Oxygen-Glucose Deprivation-Induced Injur iyn Cortical Cell Culture Doug Lobner PhD Dennis W Choi MD PhD Background and Purpose Adenosine transport inhibitors attenuate ischemic central neurona iln damag vivo bu t thee locus of this protective actio is presentln y unknown T o help

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DMEM

DMEM will work with different cell lines it can also be used with primary cells such as fibroblasts DMEM media is suitable for most types of cells including human monkey hamster rat mouse chicken and fish cells The high glucose version is well suited to high density suspension culture Shipping: Ambient Storage: 2-8 Celsius

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Tumor cell culture survival following glucose and

Objective Most glucose (and glutamine)-deprivation studies of cancer cell cultures focus on total depletion and are conducted over at least 24 h It is difficult to extrapolate findings from such experiments to practical anti-glycolytic treatments such as with insulin-inhibiting diets (with 10%–50% carbohydrate dietary restriction) or with isolated limb perfusion therapy (which usually

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Low Glucose but Not Galactose Enhances Oxidative

Background Substituting galactose for glucose in cell culture media has been suggested to enhance mitochondrial metabolism in a variety of cell lines We studied the effects of carbohydrate availability on growth differentiation and metabolism of C2C12 myoblasts and myotubes Methodology/Principal Findings We measured growth rates ability to differentiate citrate synthase and respiratory

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Cell Culture Media: A Review

So cell culture media should support maximal cell growth and sustain cell viability at increasing cell densities For the production of virus not just high cell densities are required but there must be abundant nutrients in the media to sustain virus replication after infection for example DMEM High Glucose H-21 for Lenti-X 293T cells [ 53 ]

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Ham's F10 (F

Ham#39 s Media were originally developed for the clonal growth of: Chinese Hamster Ovarynbsp (CHO) cells HeLa cells and mouse L-cells - with or without serum supplementationnbsp depending on cell types Ham#39 s F10 is a choice medium to promote human diploid cells growth white blood cells for chromosomal analysis primary rat explants and rabbit and

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Glucose Levels in Culture Medium Determine Cell Death

Cell culture and drug treatment MN9D cells were cultivated on the poly-D-Lysine (25 g/ml PDL: Sigma Chemical Co St Louis MO USA)-coated p-100 plate in Dulbecco's modified Eagle's medium (DMEM: Sigma) supplemented with heat inactivated 10% fetal bovine serum (FBS: BioWhittaker Walkersville MD USA) in an atmosphere of 10% CO 2 at 37 Prior to drug treatment culture medium was switched

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The Cytotoxic Role of Intermittent High Glucose on

Objectives Glucose fluctuations are both strong predictor of diabetic complications and crucial factor for beta cell damages Here we investigated the effect of intermittent high glucose (IHG) on both cell apoptosis and proliferation activity in INS-1 cells and the potential mechanisms Methods Cells were treated with normal glucose (5 5#x2009 mmol/L) constant high glucose (CHG) (25#

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JCI

Thus cell lines were derived on Matrigel-fibronectin–coated (100 μg/ml and 2 μg/ml respectively) (Sigma-Aldrich) culture wells in DMEM that contained 5 6 mM glucose 2% BSA fraction V (Roche Diagnostics) 50 μM 2-mercaptoethanol 10 mM nicotinamide (Calbiochem) 5 5 μg/ml transferrin (Sigma-Aldrich) 6 7 ng/ml selenite (Sigma-Aldrich

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CELL CULTURE BASICS

Cell Culture Cell culture is one of the major tools used in cellular and molecular biology providing excellent model systems for studying the normal physiology and biochemistry of cells (e g metabolic studies aging) the effects of drugs and toxic compounds on the cells

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On the Possibility of Real Time Monitoring of Glucose in

Glucose monitoring is very important in bioprocesses such as fermentation and mammalian cell culture As glucose is the major energy source for cells optimal glucose concentrations are crucial in healthy cell growth and efficient product yield In addition glucose analysis is

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Saturated Fatty Acids Synergize with Elevated Glucose to

Effect of various FFAs at different glucose concentrations on β-cell death INS 832/13 cells were cultured for 24 h in RPMI incubation medium as described in Materials and Methods containing 0 5% BSA at A Five (G5) and 20 (G20) mM glucose in the absence (control) or presence of 0 05 0 1 0 2 0 3 and 0 4 m m palmitate B 5 (G5) 11 (G11) or 20 (G20) m m glucose in the absence (control

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DMEM citations: High or low glucose?

2/16/2010DMEM citations: High or low glucose? - posted in Tissue and Cell Culture: Hello There are thousands of papers mentioning DMEM as the culture medium in their Methods without saying whether it has LOW or HIGH glucose Na-pyruvate or L-glutamine What DMEM are they referring to?

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CELL CULTURE BASICS

Cell Culture Cell culture is one of the major tools used in cellular and molecular biology providing excellent model systems for studying the normal physiology and biochemistry of cells (e g metabolic studies aging) the effects of drugs and toxic compounds on the cells

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